As a Research Assistant 2, this individual is responsible for contributing to ongoing research projects in the Center for Embryonic Cell and Gene Therapy (CECGT, http://www.ohsu.edu/xd/research/centers-institutes/embryonic-cell-gene-therapy-center/ ). A successful RA2 will perform a variety of duties, which require flexibility, judgment, and unique experience in the field of cell replacement therapy. This research center reports to the Chief Research Officer and is a critical scientific center at OHSU; thus, all employees are expected to communicate with high-level internal and external personnel and must operate with great accuracy and efficiency.
Research responsibilities include providing support to ongoing molecular biology and sequence derivation studies related to genetic, epigenetic and transcriptomic analyses of DNA (human, primate, mouse), gametes, embryos and pluripotent stem cells; participating in embryo and stem cell culture, mouse models including in vivo and in vitro experiments, maintenance of animal colonies, animal studies, cellular and molecular assays; and participating in the on-call lab schedule rotation (intermittent evening, weekend, and holiday coverage). The RA2 assists with purchasing of equipment and supplies and communicating with scientific and support staff. This position also contributes to written reports, abstracts, project proposals and grants, and presentations.
1.Research Molecular Biology and Sequence Derivation Perform various molecular techniques such as DNA/RNA/library preparation, PCR, ARMS-qPCR, PyroSequencing, Sanger sequencing, Next- Generation Sequencing (MiSeq, etc), Immunocytochemistry, Karyotyping, and other assays as deemed necessary Gel preparation, loading, and imaging Assist with analysis of sequence information and data submissions to open source databases such as the NCBI as relevant
2.Research Cell Culture and Animal Studies Assist in maintaining and derivation of fibroblast cultures Assist in maintaining ESC and iPS cell cultures, including media preparation Assist in animal-related research procedures such as mice tissue collection, derivation of mouse embryonic fibroblast cells, teratoma injection and analysis Assist in mouse colony maintenance and weekend colony maintenance as needed
3.Research General Laboratory Perform general lab upkeep and record keeping as necessary Provide support to stocking laboratory and cell culture rooms Provide support in laboratory and cell culture room cleaning schedule, autoclaving, and decontamination
4.Research Data Collection/Data Analysis/Record Keeping Collect scientific data pertinent to the research projects, keep records of information obtained during research (which may include database searches) Maintain integrity of CECGT databases
5.Report Preparation and Literature Research Prepare reports containing analytical, descriptive, and evaluative content that will be submitted for publication or for contribution to grant applications, progress reports, presentations at national and international meetings, etc. Conduct research of literature and database analysis
6.Other Projects in conjunction with the mission of the Center for Embryonic Cell and Gene Therapy and/or OHSUs Research & Innovation Office
Bachelors Degree with major courses in field of research, OR Associates Degree and 3 years of relevant work experience
Masters Degree in relevant field
3 years of relevant research experience, with demonstrated research or clinical experience in molecular biology, cell biology and genetics with laboratory experience with sequencing, cell culture and animal handling experience
Monday through Friday, commonly 8:00a 4:30p or 8:30a 5:00p position with weekend and holiday call coverage. May be required to work evenings or weekends to cover laboratory cell and embryo culture duties and laboratory animal duties. Appointee will be in contact with human, mouse, and primate tissues and fluids and will be responsible for fluid and tissue collection as related to study protocol. Training will be provided for proper handling, processes, and procedures.
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As the state's only health and research university, OHSU brings together patient care, research, education of the next generation of health care providers and scientists and community service to improve the health and well-being of all Oregonians.The university is a conduit for learning for more than 2,800 students, providing training to interns, residents and fellows in postgraduate medical and research. It is the only place in Oregon that grants doctoral degrees in medicine, dentistry and nursing. OHSU cares for more than 251,000 patients each year from around the region and takes a leadership role in contributing to solutions involving the health of the state and the nation, such as health care access and transparency, ethics and workforce shortage. Beyond providing critical health care services, quality education and cutting-edge research, OHSU also is a key economic and social force in the Northwest. With an annual operating budget of $2.06 billion and more than 14,000 employees, OHSU is Portland?s largest employer. Its size contributes to its ability to provide... many services and community support activities not found anywhere else in Oregon. For more information visit http://www.ohsu.edu/xd/about/index.cfm