The Department of Biochemistry seeks a Life Science Research Professional 1 (LSRP 1). The LSRP 1 will support a research program investigating malaria biology by performing DNA cloning and other molecular biology methods (>80% of time). S/he will be expected to design and independently construct DNA vectors that will be used in genetic experiments for various projects in the lab. S/he will also be trained in transfection of malaria parasites to generate strains with required genetic alteration. The position also entails <20% time in lab management, including ordering supplies, maintaining lab records, and adhering to safety regulations, which requires excellent organization and communication.
Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
Independently conduct experiments; maintain detailed records of experiments and outcomes.
Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
Contribute to publication of findings as needed.
Participate in the preparation of written documents, including procedures, presentations, and proposals.
Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
Assist with orientation and training of new staff or students on lab procedures or techniques.
*- Other duties may also be assignedDESIRED QUALIFICATIONS:
Degree in Molecular Biology or related field, and at least 1 year of work experience in a research laboratory using molecular biology methods
Independently perform: PCR (including primer design); restriction digest, ligation, transformation; agarose gel analysis; DNA quantification; plasmid purification (i.e. miniprep and maxiprep); troubleshooting of these methods
Understand the difference between DNA constructs used for transgene expression, protein overexpression, and gene deletion
Use of sequence analysis software to design constructs and verify Sanger sequencing results
Excellent organizational/record-keeping skills and general computer skills
Advanced DNA cloning (non-ligase dependent methods such as Gibson assembly, site-directed mutagenesis; use of g-blocks and synthesized DNA)
Advanced PCR methods (e.g. splicing by overlap extension, nested PCR)
RNA methods (e.g. construction of cDNA library)
Experience cloning AT-rich sequences
Knowledge of CRISPR-based gene disruption, transposon mutagenesis, and recombination-based integration and deletion
Website maintenance using pre-designed software
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
Ability to maintain detailed records of experiments and outcomes.
General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
Ability to work under deadlines with general guidance.
Excellent organizational skills and demonstrated ability to accurately complete detailed work.
Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
Must have correctible vision to perform duties of the job.
Position may require repetitive motion.
*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job. WORKING CONDITIONS:
May require working in close proximity to blood borne pathogens.
Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
May require extended or unusual work hours based on research requirements and business needs.
Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors bases on training and lessons learned.
Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu.
Location: School of Medicine, Stanford, California, United States Schedule: Classification Level:
Stanford is an equal opportunity employer and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other characteristic protected by law.
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Located between San Francisco and San Jose in the heart of Silicon Valley, Stanford University is recognized as one of the world's leading research and teaching institutions. Leland and Jane Stanford founded the University to "promote the public welfare by exercising an influence on behalf of humanity and civilization." Stanford opened its doors in 1891, and more than a century later, it remains dedicated to finding solutions to the great challenges of the day and to preparing students for leadership in a complex world. The University's thriving diverse community is comprised of nearly 7000 undergraduate students, 12000 graduate students, 2000 faculty members, 1900 postdoctoral scholars, and over 11,000 academic and administrative staff in seven schools including several interdisciplinary research centers and institutes. The campus spreads over 8000 contiguous acres and nearly all undergraduates live on campus. Stanford offers bachelor's and master's degrees in addition to doctoral degrees (PhD, MD, DMA and JD) plus a number of professional and continuing education programs and certifications. More at http://facts.stanford.edu and http://www.stanford.edu. Stanford is an equal empl...oyment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law. Stanford also welcomes applications from others who would bring additional dimensions to the University’s research, teaching and clinical missions.