UCSD Layoff from Career Appointment: Apply by 11/07/2019 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 11/18/2019. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Moores Cancer Center is one of just 50 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs.
The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation – from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.
Under supervision, assist with biochemical and cellular biology studies of ubiquitination, sumoylation, neddylation, autophagy, and interferon response. Assist with mouse colony management and genotyping using established PCR techniques. Work with small animals and assist with procedures such as surgery, injections and monitoring pregnancies. Assist with various cell culture techniques such as performing flow cytometry analysis of cell surface protein expression, cell propagation, contaminant testing, and cryopreservation. Assist with immunohistochemistry of frozen and fixed tissues or cells. Assist with protein biochemistry techniques including protein quantification, SDS PAGE, immunoprecipitation, and Western blotting. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication.
Theoretical knowledge of molecular biology, immunology, cell biology, and/or biochemistry.
Proven ability to perform molecular biology techniques including PCR, RT-PCR, cDNA synthesis, gene expression analysis, recombinant protein expression, RNA/DNA isolation, protein purification.
Ability to perform cell biology techniques including: mammalian cell culture, DNA transfection, cell fractionation and differential centrifugation, fluorescence- and luminescence-based bioassays.
Ability and willingness to handle and work with small animals. Experience to assist with mouse genotyping using DNA isolation, established PCR-based methods and agarose gel electrophoresis. Ability to assist in performing mouse surgeries, experiments, and injections.
Demonstrated experience in immunology, including ability to perform immunofluorescence assays, ELISA, immunoprecipitation, co-immunoprecipitation.
Ability to use histological and immunohistochemical techniques to evaluate protein localization in fixed cells or tissues.
Familiarity with basic laboratory equipment such as centrifuges, spectrophotometers, balances, pH meters, autoclaves and electrophoresis equipment.
Demonstrated ability to perform DNA and protein characterization techniques using electrophoretic analysis.
Ability to perform light, fluorescence and confocal microscopy techniques to visualize protein expression in tissue sections.
Demonstrated ability to perform sterile eukaryotic cell culture techniques.
Familiar in proper handling of chemical and biohazard materials. Familiarity with sterile techniques.
Basic understanding of computer applications such as Word, Excel and Powerpoint. Internet search and software analysis capabilities are preferred.
Strong communication skills (verbal and written) needed to interact professionally and effectively, and effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Ability to conduct biochemical analysis of ubiquitination, sumoylation, autophagy and neddylation.
Knowledge and experience with PRISM.
Employment is subject to a criminal background check.
Must be willing and able to work with hazardous materials (biological and chemical).
Must be willing and able to work with small animals.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.