As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. All of which has allowed the UW to be nationally recognized as a "Great College to Work For" for four consecutive years. The University of Washington, Department of Pathology is a broad and dynamic department that strives for a consistent level of high achievement in all of its activities. The Department offers full diagnostic services in all areas of Pathology, is a top ranked research organization as measured by research awards from the National Institutes of Health, and offers an array of specialized educational training programs.UW Medicine Pathology brings together state-of-the-art technology and highly trained, world renowned personnel to provide comprehensive diagnostic and personalized service to physicians, community hospitals, independent laboratories, and other health care organizations. Our operations are based at the University of Washington Medical Center, Harborview Medical Center, South Lake Union, Northwest Hospital and the Seattle Cancer Care Alliance. Pathology faculty members also serve at Seattle Children's Hospital, the Veteran's Administration Puget Sound Healthcare System, and the Fred Hutchinson Cancer Research Center. These collaborative efforts have generated innovative, translational studies in the basic mechanisms of disease as they apply to diagnostic pathology.The Department of Pathology, the Molecular Biophotonics laboratory has an outstanding opportunity for a full time Research Engineer/Scientist 1. We have a unique and productive pathology-engineering collaboration that uses 3D light-sheet microscopy to improve prostate cancer diagnostics. We intend to demonstrate that our technology outperforms the conventional histology approach, which requires physical sectioning of tissues onto glass slides in order to view a 2D image under an analog microscope. We are hiring a Research Scientist to work on two DOD-funded prostate cancer research projects (roughly 50% split):3D light-sheet microscopy of prostate biopsies. This project uses 1,200 archived tissue blocks from 200 patients. A "pseudo-biopsy" is cut from each tissue block. The pseudo-biopsies are immersed in a series of solvents and dyes, imaged using 3D light-sheet microscopy, and submitted for traditional histology. Duties will include cutting pseudo-biopsies, clearing and labelling the biopsies, and coordinating tissue submission to the histology core.3D micro-dissection of prostate tissue. This project will use 40 fresh prostate samples from 20 patients. Samples will be stained and cleared, imaged using 3D light-sheet microscopy, 3D micro-dissected, and then DNA and RNA will be extracted from the micro-dissected regions. Responsibilities:DNA/RNA isolation:Extract DNA and RNA from prostate samplesUse Agilent 2100 Bioanalyzer to perform quality control of extracted nucleic acidsSubmit DNA and RNA samples for next-generation sequencingLight-sheet microscopy:Dehydrate, stain, and clarify prostate samples using standardized protocols and equipment.Operate light-sheet microscope using standardized protocol.Organize and track >1,000 biopsies from 200 patients.Transport and track tissue to and from UW Medical Center.Minimum Requirements:Bachelor's degree in biology, biochemistry, chemistry, or related discipline. Competency with basic molecular biology protocols (DNA and RNA purification, electrophoresis, PCR) At least one year of wet lab related nucleic acid research experience with DNA and RNA extractionsExperience with using an Agilent BioanalyzerStrong organizational skills Ability to work independently and collaborate well with a diverse group of people and mentors. Desired:Experience with antibody labeling, e.g. immunohistochemistry, immunofluorescence, etc.Up to one year of experience in a related area or equivalent combination of education or experience. Terms and conditions for employment:Able to work a flexible schedule with occasional off hours and weekend work. Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select "Apply to this position". Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your "My Jobs" page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed. Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.